CHROMATOGRAPHY

OBJECTIVES: The objective of this experiment was to extract form foliation pigments and keep an eye on them by using the Rf values obtained from the paper chromatography technique. The ashes of the experiment was that every(prenominal) of the five listed pigments would be present in the extracted define leaf according to the Rf values. PROCEDURE/APPARATUS: The equipments expend were a 18 x 150 mm run organ pipe with stopper, graduated cylinders, Erlenmeyer flask, mortar and pestle, metric ruler, portentous jar, acetone, trivial test tube, nice brown bottle, oil color ether, plant leaf, change parsley flakes, water, glass petri dish, stapler, sand, spatula, pipette, capillary tube, Whatman deform paper, a 50 ml chromatography solvent, and goggles (to be worn at all times). The steps to performing the experiment were: 1.         Pour 50 ml of chromatography solvent into the tall jar and cover it with fractional of the petri dish. 2.           take for granted ½ teaspoonful of dried parsley flakes and ¼ teaspoon of sand in the mortar. Tear a plant leaf into small pieces, and daub it in the mortar along with the otherwise materials and grind it for 10 seconds. After that, butt the ground materials in the test tube and attract sense 5 ml of acetone. 3.         Shake for 10 seconds, and subsequently 10 minutes land 5 ml of water. Then, add 3 ml of petroleum ether and shake for 5 seconds. The pigment will in conclusion be found on the upper layer; stamp out it with a pipette and place it in a small test tube in the brown bottle. Note: immure of the remaining materials. 4.         Draw a faint pencil sway on a filter paper, 1.5 cm from one edge. Then, using a capillary tube, make 6 or 7 dots on the pencil line (repeat the step till the dots ar dark enough). 5.         Form the... If you lack to get a complete essay, order it on o ur website: OrderEssay.net

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